Images of human parasites

Authors:
Libuse Kolarova, RNDr, Ph.D.
Veronika Nemeckova, MD.
Daisy Zitova, RNDr., Ph.D.
&
Marek Bednar, MD, Ph.D.

Charles University, 3rd Faculty of Medicine, Department of Medical Microbiology,
Faculty Teaching Hospital Kralovske Vinohrady, Prague, Czech Republic


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Contents

 

Pictures of vaginal smears. Classes (0.) I.-VI. according to the classification of Jirovec et al.

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Artifact.
  • Fig. 1. Vegetable cell with amyloid granules probably from a semi-digested banana. Stained fecal film. Wheatley modification of Gomori's trichrome technique. Objective 100x.
  • Fig. 2. Bloody human feces. Leucocytes and red blood cells. Trichrome staining. Objective 100x.
  • Fig. 3. Bloody human feces. Leucocytes, one activated form with an erythrocyte inside. Trichrome staining. Objective 100x.
  • Fig. 4. Yeast cells. Thick blood smear, fixed and stained after approximately 2 hours exposure to the open air. Giemsa staining. Objective 100x.

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Blastocystis hominis
  • A unicellular organism found in the intestinal tract of travelers to tropical countries.
  • On the basis of structural and physiological examinations Blastocystis is regarded as a commensal yeast, or a protozoan, or a stramenopiles (Eukaryota), or a Proteromonas (enteric parasite of reptiles and amphibians).
  • Recently, based on molecular studies, B. hominis has been placed within the informal group, stramenopiles, a heterokontid chromista.
  • Its size is highly variable, ranging from 5 - 40 micrometers.
  • It is often associated with other parasites and bacteria.
  • Morphological forms: vacuolar, granular and amoeboid.
  • Large numbers of organisms may cause enteric disease.
  • Figs.1, 2. Fecal smears. Gomori´s trichrome (Wheatley´s modification) staining. Vacuolated forms with peripheral nuclei are present. Objective 100x.
  • Fig. 3. Cyst-like form in fecal smear (size 9 µm). Vacuolated form with peripheral nuclei. Trichrome staining. Objective 100x.
  • Fig. 4. Cyst-like form in fecal smear (size 11 µm). Vacuolated form with peripheral nuclei. Heidenhain's iron-hematoxylin staining. Objective 100x.
  • Fig. 5. Wet mount of fecal material cultured in Dobell - Laidlaw medium. A central vacuole can be seen. Unstained. Objective 40x.
  • Fig. 6. Blastocystis hominis cultivated in Dobell-Laidlaw medium. Fresh wet mount, unstained. Objective 40x.

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Leishmania sp.
  • Fig. 1. Amastigotes (round or oval bodies, 3 to5 µm in size) with large nucleus and small kinetoplast in bone marrow. Giemsa staining. Objective 100x.
  • Fig. 2. In culture, the parasite transition into flagellated promastigotes, a slender organism reaching 10 to 15 µm. Giemsa staining. Objective 100x.

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Trypanosoma sp.
  • Fig. 1. Trypomastigote stage. Thin blood smear, methyl alcohol fixation, Giemsa-Romanowski stain. Flagellates are 10 - 30 µm in length. Objective 100x.

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Giardia intestinalis (G.lamblia)
  • Fig. 1. Four elliptical cysts 8-12 x 7-10 µm in size. Stained fecal film. Wheatley modification of Gomori's trichrome technique. Objective 100x.
  • Fig. 2. One cyst containing 4 nuclei, flagella and sucking disc. Stained fecal film. Wheatley modification of Gomori's trichrome technique. Objective 100x.
  • Figs. 3, 4. Ovoid cysts with two nuclei (size 11 µm). Trichrome staining. Objective 100x.
  • Fig. 5. Trophozoites from diarrheal feces. Usually 12 x 8.5 µm in size. Stained fecal film. Wheatley modification of Gomori's trichrome technique. Objective 100x.
  • Figs. 6, 7. Unstained cysts from feces. Acute infestation. Concentration by zinc sulphate flotation (Faust's method). Objective: Fig. 6. - 100x, Fig. 7. - 40x
  • Figs. 8, 9. Unstained cysts with characteristic deformation. Fecal concentration by zinc sulphate flotation (Faust's method). Objectives 40x and 100x.
  • Figs. 10 - 12. Trophozoites from diarrheal feces. Stained fecal films. Wheatley modification of Gomori's trichrome technique. Objective 100x.
  • Fig. 13. Precystic stage of Giardia trophozoite. Wheatley modification of Gomori's trichrome technique. Objective 100x.
  • Figs. 14 - 16. Trophozoites from human diarrheal feces (size 11 - 14 µm in length). Each cell has two nuclei with a central karyosome. A ventral sucking disk is faintly visible. Trichrome staining. Objectives 100x.
  • Fig. 17. Cyst (size 13 µm) and trophozoite (size 11 µm). Trichrome staining. Objective 100x.

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Chilomastix mesnili - Cysts are usually ovoid, 8-12 x 7-10 micrometers in size. On one pole there is a tubercle.
  • Fig. 1. Stained fecal film. Wheatley modification of Gomori's trichrome technique. Objective 100x .
  • Fig. 2. Cysts stained by Heidenhain's Iron - hematoxylin. Objective 100x.

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Trichomonas vaginalis

These flagellates are 10-30 µm in length and 6-20 µm in width. Flagella, nucleus, axostyle and undulating membrane are visible.

  • Fig. 1. Vaginal smear, Acute trichomoniasis. Giemsa-Romanowski stain. Filamentous form of Lactobacillus Döderleini is present. Objective 100x .
  • Fig. 2. Trophozoites (size 14 and 15 µm in lenth). Four flagella, undullating membrane and single nucleus are visible. The dark median rod is the axostyle. Giemsa staining. Objective 100x.
  • Fig. 3. Dividing trophozoites. Giemsa staining. Objective 100x.

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Entamoeba dispar

  • Figs. 1, 2. The nonpathogenic Entamoeba dispar is morphologically identical to E. histolytica and differentiation must be based on isoenzymatic or immunologic analysis or on molecular methods. Cysts have 1 or 2 nuclei, some with chromatoidal bodies having typically blunted ends. Trichrome staining. Objective 100x.
  • Fig. 3. The uninucleated, unripe, cyst (size 12 µm) with centrally located karyosome, and fine, uniformly distributed peripheral chromatin. Chromatoidal body is visible. The smaller cyst (size 7 µm) is E. hartmanni. Trichrome staining. Objective 100x.
  • Fig. 4. Trophozoite with dark brown glycogen vacuole and pseudopodia after excystation in culture medium (Dobell-Laidlaw). Fresh wet mount, stained with Lugol's iodine solution. Objective 40x.
  • Fig. 5. Cysts (size 12 and 13 µm) with chromatoidal bodies and faintly visible nuclei. Direct preparation from fecal specimen, unstained. Objective 100x.
  • Fig. 6. Cysts (size 12 - 17 µm). Fecal concentration by zinc sulphate flotation (Faust's method), unstained. Objective 40x.
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Entamoeba histolytica (1-7). - Stained fecal film. Wheatley modification of Gomori's trichrome technique.

  • Fig. 1. Unripe, uninucleate cyst of E. histolytica f. minuta with dark chromidial bodies. Objective 100x.
  • Fig. 2. Unripe, binucleate cyst of E. histolytica f. minuta with dark chromidial bodies. Objective 100x.
  • Fig. 3. Two cysts with 4 visible nuclei. One cyst contains also elliptical chromatoidal body. Objective 100x.
  • Fig. 4. Mature quadrinucleate cyst (8-15 µm in diameter). Objective 100x.
  • Fig. 5. Trophozoite of E. histolytica forma minuta. Average cell size 10-20 micrometers. Objective 100x.
  • Figs. 6, 7. Trophozoite of E. histolytica forma magna (dysenterica). Average size, 20-30 micrometers, ingested erythrocytes seen in the endoplasm. Objective 100x.
  • Figs. 8, 9. Trophozoite of E. histolytica f. minuta after excystation in culture medium (Dobell-Laidlaw). Unstained - wet mount (10-50 micrometers). Pseudopodia and ingested rice-starch granules are visible. Objective 40x.
  • Fig. 10. Mature quadrinucleate cyst of E. histolytica f. minuta. Heidenhain's Iron - hematoxylin staining. Nuclei are clearly visible. Objective 100x.

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 Entamoeba hartmanni
  • Fig. 1. Average cyst size is 5-8 micrometers. In the left cyst (containing one nucleus) a sausage-shaped chromatoidal body is present. Stained fecal film. Wheatley modification of Gomori's trichrome technique. Objective 100x .
  • Fig. 2. Cyst (size 8 µm in diameter) with 2 nuclei and chromatoidal body. Stained fecal smear, Gomori's trichrome staining. Objective 100x.

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  Entamoeba coli - The average size of spherical cysts is 15-20 micrometers. Mature cysts contain 8 nuclei.
  • Figs. 1 - 3. Cysts with 3 to 5 visible nuclei and double wall. Stained fecal smear. Gomori's trichrome staining. Objective 100x.
  • Figs. 4 - 8. Cysts with 4 to 7 visible nuclei. Stained fecal film. Wheatley modification of Gomori's trichrome technique. Objective 100x.
  • Fig. 9. Mature cyst stained by Heidenhain's iron-hematoxylin. 7-8 nuclei are visible. Objective 100x.
  • Fig. 10. Immature uninucleate cyst. A large glycogen vacuole and peripheral nucleus are present. Wheatley modification of Gomori's trichrome technique. Objective 100x.
  • Fig. 11. Uninucleate cyst. A large nucleus and the rest of the chromidial body are visible. Wheatley modification of Gomori's trichrome technique. Objective 100x.
  • Fig. 12. Immature binucleate cyst. Glycogen vacuole and large peripheral nuclei are present. Wheatley modification of Gomori's trichrome technique. Objective 100x.
  • Fig. 13. Immature quadrinucleate cyst. Glycogen vacuole and periperal nuclei are present. Wheatley modification of Gomori's trichrome technique. Objective 100x.
  • Fig. 14. Unstained cyst (size 16 µm), 3 nuclei present. Direct preparation from fecal specimen. Objective 40x.
  • Figs. 15 - 16. Cysts (size 15 x 13 µm) typically deformed. Fecal concentration by zinc sulphate flotation (Faust's method), unstained. Objectives 40x and 100x.
  • Fig. 17. Cyst (size 15 µm in diameter). Fecal concentration by zinc sulphate flotation (Faust's method), stained with Lugol's iodine solution. Objective 100x.
  • Fig. 18. Cysts (size 16 x 17 µm). Formol ether concentration technique, unstained. Objective 40x.
  • Fig. 19. Cysts (size 15 x17 µm) with visible nuclei. Formol ether concentration technique. Stained with Lugol's iodine solution. Objective 100x.

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Endolimax nana - Cysts are 6-12 x 5-8 micrometers in size. Stained fecal film. Wheatley modification of Gomori's trichrome technique.
  • Fig. 1. Oval and spherical cysts. One cell with clearly shows 2 visible nuclei. Objective 100x.
  • Fig. 2. Two cysts (size 8 µm) with 3 visible nuclei. Objective 100x.
  • Fig. 3. Cyst with 4 visible nuclei. Objective 100x.
  • Fig. 4. Unstained cysts (size 7 to 10 µm) with characteristic deformation. Fecal concentration by zinc sulphate flotation (Faust's method). Objective 40x.

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 Iodamoeba bütschlii - Cysts are usually 8-13 x 9-15 µm in size.
  • Fig. 1. Both cysts have nuclei with eccentrically placed nucleoli and large glycogen vacuoles. Stained fecal film. Wheatley modification of Gomori's trichrome technique. Objective 100x .
  • Figs. 2, 3. Cysts with single nuclei and large endosome can be seen. Large glycogen vacuoles appear clear. Trichrome staining. Objective 100x.
  • Fig. 4. Trophozoite after excystation in Dobell-Laidlaw medium. Smear stained with Giemsa-Romanowski. Nucleus with large red nucleolus and blue cytoplasm are visible. Objective 100x.
  • Fig. 5. Spherical and oval cysts with typically cytoplasm ("rumpled paper") in fecal concentration by zinc sulphate flotation (Faust's method). Unstained. Objective 40x.
  • Figs. 6, 7. Cysts variable in shape and size with dark brown glycogen vacuoles. Fecal concentration by zinc sulphate flotation (Faust's method). Stained with Lugol's iodine solution. Objectives 40x and 100x.

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Pneumocystis jirovecii
  • Fig. 1. Crowded and collapsed empty cysts 4 µm on average. Cyst wall is blue. Bronchoalveolar lavage from a patient suffering from AIDS. Gram-Weigert stain. Objective 100x.
  • Figs. 2, 3. Cysts with irregular shape. Bronchoalveolar lavage material from immunosuppressed patient. Gram-Weigert staining. Objective 100x.
  • Fig. 4. Cysts in bronchoalveolar lavage, ciliary epithelial cells. Gram-Weigert staining. Objective 100x.
  • Figs. 5, 6. Trophozoites (size 1 to 5 µm) in bronchoalveolar lavage material. Giemsa staining. Objective 100x.
  • Fig. 7. Cysts in bronchoalveolar lavage. Toluidine blue stainig. Objective 100x.
  • Figs. 8 -10. Smear preparation of lung biopsy material. Patient died from pneumonia complications.
  • Figs. 8, 9. Numerous cysts stained blackish-brown are seen in bronchoalveolar exudate. Modified Grocott´s stain. Objective 100x.
  • Fig. 10. Honeycombed, foamy material in the alveoli, characteristic for P. carinii pneumonia. MacManus stain. Objective 100x.

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Cryptosporidium parvum
  • Fig. 1. Oocysts from diarrheal feces. Dry fecal smears were fixed in methanol and stained with using a modified Milacek technique (Milacek P, Vitovec J. Folia Parasitol. 1985;32:50 - in Czech). The oocysts appear blue-violet and are spherical, 2-5 µm on average. Objective 100x.
  • Fig. 2. Against a orange-brown background, the oocysts stand out with violet stain. Human diarrheal faeces from patient with leukaemia. Milacek technique staining. Objective 100x.
  • Fig. 3. Violet oocysts of Cryptosporidium. Morphologically similar cells to yeasts (arrows) have a similar color as the background. Milacek technique staining. Objective 100x.
  • Fig. 4. Unstained oocysts. Fecal concentration by zinc sulphate flotation (Faust's method). Objective 40x.
  • Figs. 5, 6 Unstained oocysts with sporozoites (size 5µm). Fecal concentration by zinc sulphate flotation (Faust's method). Objective 100x.

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Toxoplasma gondii
  • Fig. 1. Peritoneal exudate from white laboratory mouse, virulent strain. Tachyzoites seen both extracellularly and intracellularly within leukocytes, 8 x 2 µm on average. Giemsa-Romanowski stain. Objective 100x .
  • Fig. 2. Spherical tissue cyst with bradyzoites, cysts size are 20 - 50 µm on average. Giemsa-Romanowski stain. Objective 100x .

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Plasmodium falciparum

  • Fig. 1. A thick blood smear showing many ring forms of P. falciparum. Some leucocytes. Giemsa staining. Objective 100x.
  • Fig. 2. Plasmodium falciparum rings have delicate cytoplasm and 1 or 2 small chromatin dots. Red blood cells that are infected are not enlarged. Multiple infections of erythrocytes are more common in P. falciparum than in other species. Some leucocytes can be seen. Thin blood smear. Giemsa staining. Objective 100x.
  • Fig. 3. Two crescent shape gametocytes from a thin blood smear. Some leucocytes can be seen. Giemsa staining. Objective 100x.

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Plasmodium vivax

Figs. 1, 2. Plasmodium vivax rings and large amoeboid trophozoites have large chromatin dots and (can) show amoeboid cytoplasm. Red blood cells can be normal or enlarged up to 1 1/2x. Schüffner's dots are visible. Thin blood smear. Giemsa staining. Objective 100x.

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Plasmodium ovale

  • Fig. 1. Trophozoite of P. ovale in thick blood smear. Giemsa staining. Objective 100x.
  • Figs. 2, 3. P. ovale rings in thin blood smear. Red blood cells are normal or slightly enlarged (1 1/4x), they are round with pointed ends. Giemsa staining. Objective 100x.
  • Fig. 4. P. ovale ring shows fimbriation of the infected red blood cell. Schüffner's dots are not visible here. Thin blood smear. Giemsa staining. Objective 100x.

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Echinococcus granulosus
  • Larval stages of the parasite develop in cysts located mostly in the liver and lungs of the infected hosts (various mammals, including man). Mature cysts contain brood capsules and larvae (protoscoleces) and their surface consist of germinative and laminated layer which are produced by the parasites, and of fibrotic layer which is of host origin.
  • Fig. 1. Larval stage (protoscolex, 170 x 120 µm) isolated from a fluid of hydatid cyst; the photograph shows the hooklets on a skolex which invaginated inward of the parasite body. Fresh mount. Objective 40x.
  • Fig. 2. Evaginated scolex of the larva with hooklets and suckers. Fresh mount. Objective 40x.
  • Fig. 3. Laminated acellular parasitic membrane. Trichrome staining. Objective 20x.
  • Fig. 4. Hooklets (lenght 22 x 10 µm) on histological cross section. H&E staining. Objective 100x.

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 Echinococcus multilocularis
  • Fig. 1. Larval stages, from rodent liver, protoscoleces seen within the daughter cyst surrounded by a laminated and germinative layer. H&E staining. Objective 10x.
  • Fig. 2. Gravid proglotide (700 x 300 µm) containing numerous eggs of a mature tapeworm isolated from a cat intestine. Fresh mount. Objective 10x.
  • Fig. 3. Eggs (40 µm in diameter) from the intestinal content of a cat. Under a microscope, the eggs are undistinguishable from the eggs of other Taenia sp. Wet mount. Objective 20x.
  • Fig. 4. Transverse section of protoscoleces. H&E staining. Objective 20x.

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Diphyllobothrium latum (broad tapeworm) - The eggs are 55-75 x 35-55 µm in size, and stain light yellow. They are ovoid with an operculum and contain yolk cells.
  • Fig. 1. Fecal concentration by zinc sulphate flotation (Faust's method). Wet mount, unstained. Objective 40x.
  • Fig. 2. The egg appears oval in shape, with an operculum and a small projection at the abopercular end. Wet fresh mount. Objective 40x.
  • Fig. 3. Uterus coiled into a rosette appearance. Eggs seen inside uterus. Borax carmine staining. Objective 2x.
  • Fig. 4. Elongated head with two slit-like sucking grooves. Borax carmine staining. Objective 10x.

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Hymenolepis diminuta
  • Fig. 1. The eggs (60-79 µm in diameter) in fresh mount; the oncosphere has no polar filaments. Objective 40x.
  • Fig. 2. Larval stage (cysticercus cellulosae): invaginated scolex (1 mm in diameter) with hooks and four suckers. Borax carmine staining. Objective 10x.
  • Fig. 3. Cross section through the tegument of cysticercus cellulosae containing calcareous corpuscles. H&E staining. Objective 40x.

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 Hymenolepis nana (dwarf tapeworm)
  • Fig.1. The eggs measure 45-55 x 40-45 micrometers, are ovoid or nearly subspherical with thin shells and contain a hexacanth embryo at the centre. Fecal Zinc sulfate concentration flotation technique (Faust´s). Wet mount, unstained. Objective 40x.
  • Fig. 2. The adult tapeworm with small rounded scolex and many wide segments. Borax carmine staining. Objective 2x.
  • Fig. 3. The scolex with suckers and hooks. Objective 40x.
  • Fig. 4. The eggs inside proglottids. Borax carmine staining. Objective 40x.
  • Figgs. 5, 6. Oval colourless eggs, 44 x 37µm in size. Hooklets are present in embryos. Formol ether concentration technique. Objective 40x.

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Dipylidium caninum
  • Fig. 1. Older, larger proglottid containing characteristic egg packets that ranges from round to ovoid. Younger, smaller, proglottids below have two visible genital pores. Borax carmine staining. Objective 2x.
  • Fig. 2. Egg capsules inside a mature segment. Borax carmine staining. Objective 40x.

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 Taenia sp.
  • Fig. 1. A racemose group of eggs from a gravid proglottid (unstained). The eggs are 30 - 40 µm in length by 20 - 30 µm in width. Objective 40x.
  • Fig. 2. Group of eggs 30 µm in size. Unstained, fresh, wet mount. Objective 40x.
  • Figs. 3, 4, 5. Oval eggs with radially striated walls and visible embryonic hooklets. Unstained, fresh, wet mount. Objective 100x.

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Taenia saginata
  • Fig. 1. No hooks are present on scolex (750 µm in diameter) of the tapeworm with. Borax carmine staining. Objective 10x.

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Taenia solium
  • Fig. 1. The scolex has 4 suckers and a crown of hooks. Objective 10x.
  • Fig. 2. The eggs with thick, radially striated wall inside the segment. Objective 40x.

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 Ancylostoma duodenale / Necator americanus (hookworm disease)
  • Fig. 1. The eggs measure 60-70 x 40-50 micrometers. They are colourless and have a thin shell wall. Embryogenesis stage. The eggs of these two species are similar in size and the morfological differentiation is difficult. Fecal concentration flotation Zinc sulfate Faust´s method. Wet mount, unstained. Objective 40x.
  • Figgs. 2. 3, 4. The oval eggs (size 68 x 38µm) with visible embryo evolution. Fecal concentration flotation zinc sulphate technique, wet mount. Objective 40x.

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Ancylostoma caninum
  • Figgs. 1., 2., 3. The oval eggs (size 65 x 40µm) from dog`s faeces. Fecal concentration flotation zinc sulphate technique, wet mount. Objective 40x.
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Ascaris lumbricoides
  • Fig. 1. This egg is fertilized. It is usually 50-70 x 35-50 µm in size. Fresh stool, concentration by zinc sulphate flotation (Faust's method). Wet mount. Objective 40x.
  • Fig. 2. Unfertilized egg, measuring 80 x 50 µm, with an elongated shape. The shell is thin and the protein coat is irregular. It does not develop further. Fecal wet mount, unstained. Objective 40x.

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 Dipetalonema (Mansonella) sp. - Experimental infection.
  • Figs. 1, 2. Thick blood smear, Giemsa-Romanowski stain. Objective 100x.
  • Fig. 3. Thin blood smear, methyl alcohol fixation, Giemsa-Romanowski stain. Microfilaria measures 200 x 4.5 µm, and is unsheathed. Objective 100x.
  • Fig. 4. Larval stage (microfilaria, size 200-225 x 4.5-5 µm) of the parasite; the adult worm are usually found in connective tissues associated with organs in the abdominal cavity. Microfilariae circulate in the blood without any periodicity. Final confirmation of the filarial infection is based on detection the microfilarie in blood smears; each filarial species can be distinguished by typical morphological features, such as the presence/absence of a sheath and the position of nuclei in the specimens. Giemsa staining. Objective 100x.

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 Enterobius vermicularis (pinworm)
  • Fig. 1. Adult female (length up to 10 mm) on Scotch tape. Objective 2x.
  • Fig. 2. Unstained eggs. Specimen collected from the skin in perianal region, using Graham's cellulose adhesive tape method. Average egg size is 50-60 x 25-30 µm. Objective 20x.
  • Fig. 3. Two eggs (size 53 x 28 µm) flattened on one side with visible larvae. Sampling by Scotch tape. Objective 40x.
  • Fig. 4. Colorless egg with larva (size 55 x 32 µm) surrounded by a thick wall. Sampling by Scotch tape. Objective 100x.
  • Fig. 5. Eggs (size 50-60 x 20-30 µm) on Scotch tape. Objective 20x.
  • Fig. 6. The egg (size 58 x 28 µm) in human feces.. Fresh, wet mount. Objective 40x.
  • Figs. 7, 8. Eggs from fecal wet mount. In a stage of embryogenesis. Concentration by zinc sulphate flotation (Faust's method). Unstained. Objective 40x.
  • Fig. 9. Egg (size 56 x 27µm). Fecal concentration by zinc sulphate flotation (Faust's method), wet mount. Objective 40x.
  • Fig. 10. The adult female worm in human feces. Fresh, wet mount. Objective 2x.
  • Fig. 11. Anterior of an adult worm in human feces. Broad esophagus is visible. Fresh, wet mount. Objective 2x.
  • Figs. 12, 13, 14. Video - Moving larvae. Objectives 40x and 100x.
  • Fig. 15. Transverse section through the body of a female (diameter 200 x 170 µm) in the human appendix; illustration of the thin cuticle, muscle cells and lateral chords. H&E staining. Objective 40x.

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Loa loa
  • Fig. 1. Larval stage (microfilariae, size 250-300 x 8-10 µm) of the parasite circulates in the peripheral blood with a diurnal periodicity (microfilaria diurna,); the adult parasite reside and wander freely through the connective tissues (causing Calabar swelling) of the ocular conjuctivae. Final confirmation of the filarial infection is based on detection the microfilarie in blood smears; each filarial species can be distinguished by typical morphological features such as the presence/absence of a sheath and the position of body nuclei in the specimens. Giemsa staining. Objective 100x.

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1 click to enlarge; İKolarova
Onchocerca volvulus
  • Fig. 1.Transverse section of an adult female in human skin. When the parasite locate in the skin, the inflammatory reaction results in the formation of nodules and the microfilariae can be detected in the surrounding tissue using histological techniques. Staining shows typical morphological features - moderately thick cuticle, well developed hypodermis, weak musculature and the uterine branches containing developing microfilariae (254-332 x 6-8 µm). H&E staining. Objective 10x.

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1 click to enlarge; İKolarova
 Strongyloides stercoralis
  • Figs. 1, 2. Unstained larval parasitic stage (200 - 300 x 15 - 20 µm). The larvae have a shorter esophagus with two swellings. Concentration by zinc sulphate flotation (Faust's method). Objective 40x.
  • Fig. 3. Transverse section through the body of adult females and larvae in the human intestine. Giemsa staining. Objective 20x.

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1 click to enlarge; İZitova
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3 click to enlarge; İKolarova
 Toxocara canis
  • Figs. 1 - 7. The spherical eggs (size 75 - 90 µm in diameter) with developing larva (from dog feces) surrounded by a thick shell. Fresh, wet mount. Objectives 40x.

  • Fig. 8. The egg on the right is fertilized but not yet embryonated, while the egg on the left contains a well developed larva. Fresh, wet mount. Objective 20x.

  • Figs. 9, 10. Process of larva hatching. Fresh, wet mount. Objective 40x.

  • Figs. 11, 12. Free larva released from the egg. Fresh, wet mount. Objective 20x.

  • Fig. 13. Video - Larva being released from an egg.

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Trichinella spiralis
  • Fig. 1. Parasite larvae, reaching up to 1 mm in length, encapsuled in striated muscle tissue; the cyst (450 x 160 µm) is typically elongated or spindle shaped. Since 1972, Trichinella species with specific biological and behavioral characteristics different from T. spiralis have been described, e.g. T. brittovi, T. nativa, T. nelsoni, T. psedospiralis. Histology of a muscle biopsy showing a cross section of a larva. Trichrome staining. Objective 20x.
  • Fig. 2. As seen in transverse section, a stichocyte, a feature that readily identifies the parasite. H&E staining. Objective 40x.

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1 click to enlarge; İKolarova
2 click to enlarge; İKolarova
Trichuris trichiura (whipworm).
  • Fig. 1. The lemon-shaped eggs have a thick shell and polar plugs. They are stained yellowish-brown by the bile pigment in the feces. They measure 50-54 x 22-23 µm. Concentration by zinc sulphate flotation (Faust's method). Seen in the stage of embryogenesis. Objective 40x.
  • Fig. 2. The yellow-brown egg (size 59 x 22 µm) with two polar unstained mucoid plugs, has a characteristic barrel shape. Fecal concentration by zinc sulphate flotation (Faust's method). Objective 100x.
  • Fig. 3. Typical egg (size 54 x 22 µm). Trichrome staining. Objective 100x.

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1 click to enlarge; İZitova
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Schistosoma sp.
  • Fig. 1. Egg (size 137 x 62 µm) with terminal spine, from stool specimen of a traveler who went swimming in Lake Malawi. Formol ether concentration technique. Objective 20x.
  • Fig. 2. Egg (size 135 x 55 µm) with terminal spine from stool specimen of a traveler who went swimming in Lake Malawi. Formol ether concentration technique. Objective 40x.

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1 click to enlarge; İNemeckova
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Schistosoma haematobium
  • Fig. 1. Pale yellow-brown eggs measuring approximately 145 x 55 µm. They are oval in shape with a small spine at one end (terminal spine). Fresh, wet mount of urine sediment. Objective 40x.
  • Fig. 2. Free ciliated miracidium released from an egg. Fresh, wet mount. Objective 40x.
  • Figs. 3, 4, 5. Numerous large-size ova with terminal spine (arrows), each containing multinucleated miracidium. Specimen taken from bladder wall. H&E staining. Objective 40x

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Schistosoma japonicum
  • Figs. 1, 2. Oval eggs, measuring approximately 90 x 65 µm, containing developed miracidium. The very small hook-like spine is not seen. Objective 40x.

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1 click to enlarge; İNemeckova
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 Schistosoma mansoni
  • Figs. 1, 2. Oval egg (size approximately 150 x 60 µm) with typical lateral spine, isolated from the liver of an experimentally infected mouse. Objective 40x.
  • Fig. 3. Viable egg from a stool specimen of woman from Angola. Well developed miracidium and lateral spine are visible. Formol ether concentration technique. Objective 40x.
  • Fig. 4. The same specimen but the egg is turned, therefore lateral spine is not visible. Objective 40x.
  • Fig. 5. Ciliated motile miracidium hatched from an egg. Fresh, wet mount. Objective 40x.
  • Fig. 6. The remaining shell of the egg after hatching miracidium. Fresh, wet mount. Objective 40x.
  • Fig. 7. Eggs, in liver tissue, surrounded by a fibrotic granuloma. H&E staining. Objective 20x.
  • Fig. 8. Eggs in liver tissue. PAS staining. Objective 40x.
  • Fig. 9. Eggs (size 110-175 x 45-40 µm) surrounded by granulomatous reaction in a native fresh mount prepared by compressing mouse liver tissue between two slides. Objective 20x.
  • Fig. 10. Eggs in the wall of the mouse rectum; histology shows collapsed eggs in the granulomas. PAS staining. Objective 40x.
  • Fig. 11. Adult flukes: males and females in copula, specimen from mouse liver vein; females reside within the gynecophoral canal of males where the tegument shows irregular tuberculations. H&E staining. Objective 20x.

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 Trichobilharzia regenti
  • Fig. 1. The larval stages (cercariae) released from water snails (intermediate host) in beaker. Final hosts of this avian schistosoma are water birds. The penetration of its cercariae causes a dermatitis which is usually accompanied by an intense itching. In humans, however, the larvae never mature and die at various intervals following infection.
  • Fig. 2. Cercaria with forked-tail and ventral sucker (arrow). Unstained. Objective 10x.
  • Fig. 3. Cercaria with forked-tail and "eye-spots" on head. Lugol's iodine solution. Objective 10x.

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Neotrombicula autumnalis.
  • Fig. 1. Trombiculid harvest mites (unstained). Hexapode larval stage, about 300 micrometers in length. Objective 2x.

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1 click to enlarge; İZitova
Sarcoptes scabiei (itch mites, sarcoptid mites)

Unstained, with short legs. They produce excavated tunnels in the epidermis and cause scabies.

  • Fig. 1. Adult male, 0,2 - 0,25 mm in length. Objective 20x.
  • Fig. 2. Adult female, 0,3 - 0,4 mm in length. Objective 20x.
  • Fig. 3. Adult female and egg of the mite containing a larva. Objective 10x.
  • Fig. 4. The eggs with larvae. Objective 10x.
  • Fig. 5. Larva hatching from the egg. Objective 10x.
  • Fig. 6. The empty egg. Objective 10x.

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 Pulex irritans
  • Fig. 1. Female (human flea), unstained. The species vary from 1,5 - 4,0 mm in length. Objective 2 x.

Zoological Institute, St.Petersburg FLEAS HOME PAGE

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Ctenocephalides canis (cat flea)
  • Fig. 1. Male and female. Unstained. Objective 2x.

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Ctenocephalides felis (cat flea)
  • Fig. 1. female.

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Tunga penetrans (sand flea). The remnants of abdomen with large numbers of eggs, discharged from the skin lesion. The oval eggs are 550-600 x 350-370 micrometers in size. Unstained. Imported infection from Central Africa.
  • Fig.1. Objective 10x
  • Fig.2. Objective 2x

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Pediculus capitis (head louse)

Unstained.

  • Fig. 1. Adult female, 2.5 - 3 mm in length. Objective 2x.
  • Fig. 2. Adult female louse. Objective 2x.
  • Fig. 3. Adult male, 2.4 - 2.6 mm in length. Objective 2x.
  • Fig. 4. Adult male louse. Objective 2x.
  • Fig. 5. Female abdominal region with genitalia. Processes of the telson are wider, therefore the interspace is narrower. Triangle-like gonapophysis is seen medially above the telsons. Objective 10x.
  • Fig. 6. Membranous onychium (arrow) typical of head louse. Objective 40x.

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Pediculus humanus (body louse)

Unstained.

  • Fig. 1. Adult male, 3 - 3.2 mm in length. Objective 2 x.
  • Fig. 2. Adult female, 3.5 - 4.2 mm in length. Objective 2 x.
  • Fig. 3. Female abdominal region with genitalia. Processes of the telson are narrower, therefore the interspace is wide. Falciform gonapophysis is seen medially above the telsons. Objective 10x.

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Phthirus pubis (pubic or crab louse)

Unstained.

This louse is shorter than that of body or head lice, and grey in color. The claws on the legs are very large. Location: pubic hair, eyelashes. Venereally transmitted.

  • Fig. 1. Adult male, 0.8-1.0 mm in length. Objective 2x.
  • Fig. 2. Adult male abdominal region with genitalia. Objective 10x.
  • Fig. 3. Adult female, 1.0-1.2 mm in length. Objective 2x.
  • Fig. 4. Adult female abdominal region with genitalia. Objective 10x.

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Pictures of vaginal smears

Pictures of vaginal smears - classes 0., I., II., III., IV., V., VI. according to the classification of Jirovec et al.*

* Jirovec O, Peter R, Malek I: Neue Klassifikation der Vaginalbiocoenose auf sechs Grundbilder. Gynaecologia 126:2,77-99 (1948) (in German)

This variant of the vaginal smear classification is commonly used only in the Czech Republic. It is a screening technique and does not replace culturing vaginal material. In other countries similar methods are used. 

O. - Quiescence

Fig. 1. Premenstrual (child´s) or postmenopausal (resting). Large round epithelial cells, solitary leukocytes and bacteria, Lactobacillus is not present.

1 click to enlarge; İZitova
I. - Healthy, normal
  • Fig. 1. Döderlein flora with rod-shaped lactobacilli (from healthy fertile woman): Epithelial cells, Döderlein´s lactobacillus, other bacteria and leukocytes. Objective
  • Fig. 2. Epithelial cells, few leucocytes. Döderlein`s lactobacillus is visible as detritus. Giemsa-Romanowski staining. Objective 10x.
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II. - Non-purulent bacterial colpitis.
  • Fig. 1. Epithelial cells, solitary leukocytes, mixture of bacteria, lactobacillus is not present. Objective 100x.
  • Fig. 2. Epithelial cells, mixture of bacteria. Giemsa-Romanowski staining. Objective 100x.
  • Fig. 3. Epithelial cells, leucocytes. Bacteria are visible as detritus. Giemsa-Romanowski staining. Objective 10x.
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  II.b.- Non purulent bacterial colpitis with vibrio-like rods.

Fig. 1. Epithelial cells, mixture of bacteria and crescent-shaped vibriae. Objective 100x.

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III. - Purulent bacterial colpitis.
  • Fig. 1. Epithelial cells, numerous leukocytes, mixture of bacteria, no lactobacillus present. Objective 100x.
  • Fig. 2. Epithelial cells, many leucocytes. Bacteria are visible as dots. Giemsa-Romanowski staining. Objective 10
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IV. - Gonococcus infection (subsequent cultivation was positive).
  • Figs. 1, 2. Gonorrhoic vaginal or urethral discharge. Epithelial cells, numerous leukocytes, intracellular diplococci, other bacteria seen sporadically.
  • Fig. 3. Urethral smear. Epithelial cells, numerous leucocytes. Intracellular and extracellular diplococci. Giemsa-Romanowski staining. Objective 100x.
  • Fig. 4. Urethral smear. Epithelial cells, numerous leucocytes. Giemsa-Romanowski staining. Objective 10x.
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V. - Trichomonas infection.
  • Fig. 1. Vaginal discharge. Epithelial cells, leukocytes, filamentous form of Döderlein´s lactobacillus, other bacteria. Flagella, nucleus, axostyle and undulating membrane are visible in trophozoites.
  • Fig. 2. Vaginal smear. Leucocytes. Trophozoites with purple nuclei. Giemsa-Romanowski staining. Objective 100x.
  • Fig. 3. Vaginal smear. Leucocytes, some bacteria. Trophozoites with purple nuclei and powder blue cytoplasm. Giemsa-Romanowski staining. Objective 100x.
  • Fig. 4. Vaginal smear. Leucocytes, some bacteria. Spherical trophozoites 19µm in size. Giemsa-Romanowski staining. Objective 100x.
  • Fig. 5. Vaginal smear. Epithelial cells, numerous leucocytes. Spherical trophozoites (arrows). Giemsa-Romanowski staining. Objective 10x.
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VI.

Vulvovaginal candidiasis (monilial infection)

  • Fig. 1. Pseudohypha, leucocytes, epithelial cells, Döderlein`s lactobacillus, other bacteria. Objective 100x.
  • Fig. 2. Vaginal smear. Epithelial cells, several leucocytes. Pseudohyphae and germinated blastokonidia. Giemsa-Romanowski staining. Objective 100x.
  • Fig. 3. Vaginal smear. Epithelial cells, leucocytes. Pseudohyphae (arrows). Giemsa-Romanowski staining. Objective 100x.

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1 click to enlarge; İZitova
2 click to enlarge; İNemeckova
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Authors: Libuse Kolarova - Veronika Nemeckova - Daisy Zitova - Marek Bednar

English editing: Thomas Secrest

This project was supported by Grants FRVS (Grant Agency of the Czech Ministry of Education) 1937/2001 and 884/2006.

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